Preheat the oven to 350 degrees F. Spray loaf pan with non stick cooking spray.
In a large bowl, combine beef, bread crumbs, onion, peppers, garlic, italian seasoning, salt and pepper until combined.
Pack the meatloaf mixture tightly into the prepared loaf pan.
Spread ketchup on top of the loaf and bake for 45 minutes, until internal temperature reaches 165 degrees F on a meat thermometer inserted into the center of the loaf.
Remove the meatloaf from the oven and cool for 15 minutes before serving.